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This book is Free on June 15, Are these books no longer free? Owing to the presence of lead chromate in formulation and retention of flexible casting properties when catalyzed and set  , Microfil provides a number of characteristics amenable to microCT imaging of vascular networks in prepared specimens.
Materials and Methods Small animal preparation and perfusion All procedures were conducted in accordance with institutional, US and international regulations and standards on animal welfare. Ethical approval was granted by the UCLA animal research committee animal protocol number Surgical procedures were enacted to prepare the specimen for a postmortem intracardial perfusion.
Briefly, a midline incision was made from pubic symphysis to xiphoid process.
The diaphragm was severed peripherally to expose the thoracic cavity. The heart was cannulated distally, at the apex of the left ventricle, and the right atrium was severed to provide outflow. At the conclusion of the procedure, specimens were loosely wrapped in aluminum foil and placed in the refrigerator to cure overnight.
Scan time ranged from 7. File sizes ranged from 1. Scan time was 4 hours.
File size was 3. Results Microfil imaged by microCT shows in-situ filling of vasculature of preclinical specimen organs in three spatial dimensions After curing, MicroFil perfused organs were scanned at parameters appropriate for deriving fine vascular detail.
As shown in Figure 1 , the filled lumen of the kidney vasculature is made visible in volume renderings A—D. Because region of interest data is of most concern to individual researchers, it is possible to digitally isolate focal areas for preferred observation.
Panel A of Figure 1 shows large vessel signal for an adult mouse kidney in a sagittal orientation. Panels B—D show perfused mouse kidneys rendered in various orientations, detailing finer vasculature features, including those of the mouse adrenal gland.